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Nonlinear Fourier-transform spectroscopy using ultrabroadband pulses for measurement of photobleaching spectra of fluorescent proteins

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Abstract

We present the measurement of photobleaching spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy using ultrabroadband femtosecond pulses. Photobleaching of two-photon excited fluorescent molecules occurs through one-photon excited-state absorption.

© 2014 Optical Society of America

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