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Conferences > CLEOPR > 2015 > 27P > 27P_67
  • 2015 Conference on Lasers and Electro-Optics Pacific Rim
  • (Optica Publishing Group, 2015),
  • paper 27P_67

Fluorescence lifetime determination of photo-bleaching correlation

Won Sang Hwang, Young Sik Song, and Dug Young Kim

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Abstract

FLIM is relatively stable about photo-bleaching effect. In case of dye has multi-exponential decay property, fluorescence lifetime can be affected by photo-bleaching. Such lifetime variation can cause critical error in many FLIM applications. If lifetime is changed by photo-bleaching, we can’t measure accurately donor lifetime with acceptor. It is difficult to separate lifetime variation by photo-bleaching and FRET. For this reason, we carry out the experiment to know influence of such fluorescence intensity loss on fluorescence lifetime.

© 2015 IEEE

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