Two-photon holographic microscopy (2PHM) offers the advantage of simultaneous multi-site excitation in three dimensions. This is useful for studies of neuronal signal integration, which require multiple controlled synaptic inputs delivered simultaneously onto dendritic trees. In this work, we holographically split a single femtosecond pulse-laser in order to project multiple foci onto the neuron. At each focus, two-photon photolysis of caged neurotransmitter molecules, which bind to receptors and mimic synaptic transmission, is performed, thereby allowing measurement of how neurons integrate multiple synaptic inputs.

© 2011 AOS

PDF Article
More Like This
Holographic multi-site Ca2+ imaging along thin dendrites of cortical pyramidal neurons

Michael L. Castanares and Vincent R. Daria
JTh5A.3 Clinical and Translational Biophotonics (Translational) 2018

Efficient holographic multi-site two-photon fluorescence for functional calcium imaging of neuronal circuits

Michael Lawrence Castanares, Vini Gautam, Hans Bachor, and Vincent R. Daria
DT4G.2 Digital Holography and Three-Dimensional Imaging (DH) 2016

Light-neuron interactions: key to understanding the brain

Vincent R Daria
BrT4B.3 Optics and the Brain (BRAIN) 2015


You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
Login to access Optica Member Subscription