Adaptive optics for two-photon and harmonic eneration microscopy

Anisha Thayil; Alexander Jesacher; Tony Wilson; Martin J. Booth

doi:10.1364/CLEO.2010.CWD5

Conference on Lasers and Electro-Optics 2010
OSA Technical Digest (CD) (Optica Publishing Group, 2010),
paper CWD5
•https://doi.org/10.1364/CLEO.2010.CWD5

Adaptive optics for two-photon and harmonic eneration microscopy

Anisha Thayil, Alexander Jesacher, Tony Wilson, and Martin J. Booth

Conference on Lasers and Electro-Optics 2010

San Jose, California United States
16–21 May 2010
ISBN: 978-1-55752-889-6

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Multiphoton Microscopy (CWD)

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A. Thayil, A. Jesacher, T. Wilson, and M. J. Booth, "Adaptive optics for two-photon and harmonic eneration microscopy," in Conference on Lasers and Electro-Optics 2010, OSA Technical Digest (CD) (Optica Publishing Group, 2010), paper CWD5.

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Abstract

Specimen-induced aberrations frequently affect image quality in high-resolution microscopes. We apply adaptive optics to correct aberrations in two-photon fluorescence, and second and third harmonic microscopes. In particular, this is applied to imaging of mouse embryos.

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