Abstract

Fluorescence lifetimes are sensitive to local physical conditions and insensitive to artifacts affecting intensity based measurements, providing a complementary source of contrast for fluorescence microscopy.¹ While lifetime microscopy is well-developed at visible wavelengths (e.g., fluorescence resonance energy transfer between exogenous fluorophores), FLIM of endogenous fluorophores is less developed with many potential uses (e.g., biomedical diagnostics).²

© 2002 Optical Society of America