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Optica Publishing Group
  • Conference on Lasers and Electro-Optics
  • OSA Technical Digest (Optica Publishing Group, 1996),
  • paper CTuH2

Diode-pumped lasers for two-photon fluorescence microscopy

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Abstract

We report the use of diode-pumped mode-locked lasers for generating two- photon fluorescence microscopy (TPFM) images, TPFM offers several advantages over the more conventional confocal fluorescence microscopy,1,2 namely improved depth penetration through samples, confinement of dye bleaching to the focus area, and a reduction in the background fluorescence levels caused by out-of-focus fluorescence. In addition, the absorption confinement reduces phototoxicity for live samples. To date argon-ion-pumped Ti:sapphire lasers have been used to generate TPFM images, however the requirement of commercial systems is a compact device with a long lifetime and low running costs. This implies the use of diode-pumped systems.

© 1996 Optical Society of America

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