The Tabor and Richardson strategy for enzymatic chain termination sequencing of DNA using relative peak intensity has been adapted to capillary gel electrophoresis with laser-induced fluorescence detection (CGE/LIF). This approach to DNA sequencing involves the use of only a single fluor and results in significant reduction in the time required to determine a DNA sequence without the use of highly complicated and expensive instrumentation.

© 1992 Optical Society of America

PDF Article
More Like This
High-sensitivity DNA analysis with laser- excited confocal fluorescence scanning

Richard Mathies
JMA1 Conference on Lasers and Electro-Optics (CLEO) 1994

Fluorescence Monitoring of Microchip Capillary Electrophoresis Separation with Monolithically Integrated Optical Waveguides

C. Dongre, R. Dekker, H. J. W. M. Hoekstra, R. Martinez-Vazquez, R. Osellame, R. Ramponi, G. Cerullo, R. van Weeghel, G. A. J. Besselink, H. H. van den Vlekkert, and M. Pollnau
CTuM5 Conference on Lasers and Electro-Optics (CLEO) 2009

Automated DNA sequence using fluorescence detection

THY1 Conference on Lasers and Electro-Optics (CLEO) 1988