A Mesoscopic Oblique Plane Microscopy (OPM) Platform for Centimeter Scale Volumetric Imaging

Wenjun Shao; Kivilcim Kilic; Wenqing Yin; Gregory Wirak; Xiaodan qin; Hui Feng; David Boas; David Boas; Christopher V. Gabel; Ji Yi

doi:10.1364/BRAIN.2021.BM3B.1

Biophotonics Congress 2021
OSA Technical Digest (Optica Publishing Group, 2021),
paper BM3B.1
•https://doi.org/10.1364/BRAIN.2021.BM3B.1

A Mesoscopic Oblique Plane Microscopy (OPM) Platform for Centimeter Scale Volumetric Imaging

Wenjun Shao, Kivilcim Kilic, Wenqing Yin, Gregory Wirak, Xiaodan qin, Hui Feng, David Boas, Christopher V. Gabel, and Ji Yi

Optics and the Brain 2021

Washington, DC United States
12–16 April 2021
ISBN: 978-1-943580-85-9

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Large-scale Volumetric Single-photon Imaging (BM3B)

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W. Shao, K. Kilic, W. Yin, G. Wirak, X. qin, H. Feng, D. Boas, C. V. Gabel, and J. Yi, "A Mesoscopic Oblique Plane Microscopy (OPM) Platform for Centimeter Scale Volumetric Imaging," in Biophotonics Congress 2021, C. Boudoux, K. Maitland, C. Hendon, M. Wojtkowski, K. Quinn, M. Schanne-Klein, N. Durr, D. Elson, F. Cichos, L. Oddershede, V. Emiliani, O. Maragò, S. Nic Chormaic, N. Pégard, S. Gibbs, S. Vinogradov, M. Niedre, K. Samkoe, A. Devor, D. Peterka, P. Blinder, and E. Buckley, eds., OSA Technical Digest (Optica Publishing Group, 2021), paper BM3B.1.

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Abstract

We implemented a novel optical design to allow using low numerical aperture objective lens in OPM and achieved centimeter-scale volumetric imaging. It has great potential in large specimen imaging such as whole-brain imaging of mice.

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