Abstract
We present an intravital imaging system based on three-photon excitation, indirect adaptive optics and active electrocardiogram gating which achieves near-diffraction-limited resolution up to 1.2mm in the mouse brain. We demonstrate the capability of our technique to study spine morphology and neuroglia function non-invasively throughout an entire cortical column and in the hippocampus in the mouse brain in-vivo.
© 2021 The Author(s)
PDF Article | Presentation VideoMore Like This
Antoine Hubert, Fabrice Harms, Sophia Imperato, Vincent Loriette, Cynthia Veilly, Xavier Levecq, Georges Farkouh, François Rouyer, and Alexandra Fragola
NM2C.4 Novel Techniques in Microscopy (NTM) 2021
David Sinefeld, Tianyu Wang, Mengran Wang, Hari P. Paudel, Thomas G. Bifano, and Chris Xu
ATu4O.2 CLEO: Applications and Technology (CLEO:A&T) 2016
Yao Zheng (speaker), Wei Gong, and Ke Si
T4A.3 International Conference on Photonics and Imaging in Biology and Medicine (PIBM) 2017