3-Photon Calcium Imaging of Deep Cortical Layers for Functional Connectomics

Kevin Takasaki; Josh Larkin; Reza Abbasi-Asl; Dan Denman; Dan Millman; Saskia de Vries; Marc Takeno; Nuno M. da Costa; R. Clay Reid; Jack Waters

doi:10.1364/BRAIN.2019.BM4A.4

Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP)
OSA Technical Digest (Optica Publishing Group, 2019),
paper BM4A.4
•https://doi.org/10.1364/BRAIN.2019.BM4A.4

3-Photon Calcium Imaging of Deep Cortical Layers for Functional Connectomics

Kevin Takasaki, Josh Larkin, Reza Abbasi-Asl, Dan Denman, Dan Millman, Saskia de Vries, Marc Takeno, Nuno M. da Costa, R. Clay Reid, and Jack Waters

Optics and the Brain 2019

Tucson, Arizona United States
14–17 April 2019
ISBN: 978-1-943580-54-5

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Functional Microscopy (BM4A)

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K. Takasaki, J. Larkin, R. Abbasi-Asl, D. Denman, D. Millman, S. de Vries, M. Takeno, N. M. da Costa, R. C. Reid, and J. Waters, "3-Photon Calcium Imaging of Deep Cortical Layers for Functional Connectomics," in Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP), OSA Technical Digest (Optica Publishing Group, 2019), paper BM4A.4.

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Abstract

2-photon image quality degrades in volume-labeled tissue due to high background fluorescence. We apply 3-photon imaging to measuring functional responses in deep layers of primary visual cortex in pan-excitatory transgenic mice expressing GCaMP6s.

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