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Conferences > BRAIN > 2015 > BrM2B > BrM2B.3

Fast, Volumetric Imaging of In Vivo Mouse Brain with Swept Confocally Aligned Planar Excitation (SCAPE) Microscopy

Venkatakaushik Voleti, Matthew B. Bouchard, Clay Lacefield, Randy Bruno, and Elizabeth M. C. Hillman

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Abstract

We demonstrate the use of SCAPE microscopy to image both neural activity via GCaMP and vascular hemodynamics in the awake behaving mouse brain at 10-20 volumes per second with cellular resolution over large fields of view. The performance of SCAPE is compared to in-vivo two-photon microscopy.

© 2015 Optical Society of America

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