Abstract

A simulation experiment for 3-D imaging exogenous fluorescinated antibodies that specifically bind to infiltrating lymphocytes in minor salivary glands was carried out. Rhodamine targets embedded in a Delrin disk were covered by Delrin slabs of various thickness. Surface images were obtained for a different target depths and radial distances from laser excitation to target fluorophore. These images were analyzed and compared to theoretical calculations using previously determined scattering and absorption coefficients of the Delrin. Good agreement between experiments and theory was achieved.

© 1996 Optical Society of America