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Absolute Enumeration of Rare Cell Types in Peripheral Blood Using Laser Induced Fluorescence and Volumetric Microscopy

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Abstract

This paper describes a novel technique for the absolute enumeration of dye-labeled cells using laser-induced fluorescence and volumetric microscopy. This technique has been used for the enumeration of CD4+ and CD8+ lymphocytes, CD34+ stem cells, and quality checking of leukocyte-reduced blood products.

© 1996 Optical Society of America

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