Rapid fluorescent labeling combined with optical sectioning and H&E-rendering algorithms can provide histological imaging of surgical specimens more rapidly than frozen sections by avoiding the need to freeze tissue and physically cut slides. Using a novel protocol that enables precise coregistration of nonlinear microscopy (NLM) and diagnostic frozen sections (FSA) produced during Mohs surgery, we present the results of a study comparing the relative accuracy of NLM and FSA.

© 2019 The Author(s)

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