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Conferences > BODA > 2015 > OT4D > OT4D.3

TIRF-FLIM imaging of membrane viscosity using artificial membranes and molecular rotors

N. D. Geoghegan, M. R. Dent, D. J. Paterson, A. Glidle, N. J. Brooks, M. K. Kuimova, and J. M. Cooper

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Abstract

A new method for imaging membrane viscosity is presented using molecular rotors and a combined TIRF-FLIM approach. Lifetimes of artificial membranes of pure DOPC and DPPC were measured and DOPC over a range of temperatures.

© 2015 Optical Society of America

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