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Swept confocally-aligned planar excitation (SCAPE) microscopy for high speed volumetric imaging in behaving animals

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Abstract

SCAPE combines light-sheet optical sectioning with confocal de-scanning to produce very high-speed 3D imaging in a versatile single-objective geometry. SCAPE can image awake behaving mouse brain, zebrafish heart and crawling Drosophila larvae at 10-20 volumes-per-second.

© 2015 Optical Society of America

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