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Non-Negative Matrix Factorization to remove autofluorescence of tissues and improve FDOT

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Abstract

Autofluorescence of biological tissues limits deep fluorescent markers detection. A spectroscopic approach and a blind source separation method are explored to remove the autofluorescence signal. We show how this preprocessing improves Fluorescent Diffuse Optical Tomography.

© 2010 Optical Society of America

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