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Snapshot Image Mapping Spectrometer (IMS) for Hyperspectral Fluorescence Microscopy

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Abstract

Image Mapping Spectrometer (IMS) is a snapshot hyperspectral imaging technique based on redirection and dispersion of image zones. Recently, two generations of IMS systems have been built in our group: a low-sampling IMS prototype with datacube size 100×100×25 (x, y, λ) and a high-sampling IMS with datacube size 285×285×60 (x, y, λ). In this paper, the operating principle of IMS is illustrated and preliminary cell fluorescent images are shown. Experiment results demonstrate that IMS has a promising perspective in hyperspectral biomedical imaging applications.

© 2010 Optical Society of America

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