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Live cell refractometry using Hilbert phase microscopy

Niyom Lue, Gabriel Popescu, Takahiro Ikeda, Kamran Badizadegan, Ramachandra R. Dasari, and Michael S. Feld

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Abstract

Using Hilbert phase microscopy developed in our laboratory, we determined the average refractive index of suspended epithelial cells. The technique requires no preparation and can be easily automated and integrated with flow cytometry.

© 2006 Optical Society of America

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