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Probing the local environment of green fluorescent protein (GFP) with fluorescence lifetime imaging (FLIM) and time-resolved fluorescence anisotropy imaging (tr-FAIM)

K. Suhling, D.M. Davis, D. Phillips, J. Siegel, S. Lévêque-Fort, S.E.D. Webb, and P.M.W. French

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Abstract

Wide-field time-domain fluorescence lifetime imaging and time-resolved fluorescence anisotropy imaging of green fluorescent protein can be used to probe the biophysical environment of specific proteins.

© 2002 Optical Society of America

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