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3D-Photoacoustic Imaging of Blood Vessels

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Abstract

We used Photoacoustics to image optically absorbing structures (blood vessels) in tissue samples. With 532 nm light depths down to 6-9 mm were reached. Samples were a 10 % solution of Intralipid-10% or real tissue (chicken breast), containing capillaries with blood or a variable dilution of Evans blue. Small PVdF piezoelectric hydrophones were used for detection in scanning mode. The sensitivity of the technique was demonstrated by the detection of two single red blood cells situated on a glass plate. The lateral resolution is limited by the diameter of the detector (200 pm in our case). The depth resolution is about 10 μm.

This study was undertaken to develop Photoacoustic Tomography of tissue for the localisation of blood vessels, e.g. for the detection of blood concentrations (angiogenesis) around tumors.

© 1998 Optical Society of America

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