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High-Speed and High-Resolution Raman Imaging of Biological Molecule Using Line Illumination

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Abstract

We have developed a Raman imaging technique that can visualize biological events in living cells using vibrational fingerprints of molecules. Using the resonance Raman effect, we specifically observed the intracellular activities of heme proteins such as cytochrome c without labeling. The sensitivity of Raman scattering to the redox state of heme proteins provides additional insight into the role of proteins from a chemical perspective.

© 2021 The Author(s)

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