Characterization and bioanalytical application of innovative plasmonic nanostructures

D. Cialla; D. Cialla; K. Weber; K. Weber; R. Boehme; U. Huebner; H. Schneidewind; M. Zeisberger; R. Mattheis; J. Popp; J. Popp

doi:10.1364/ACP.2011.83110J

Optical Sensors and Biophotonics
Proceedings of SPIE (Optica Publishing Group, 2011),
paper 83110J
•https://doi.org/10.1364/ACP.2011.83110J

Characterization and bioanalytical application of innovative plasmonic nanostructures

D. Cialla, K. Weber, R. Boehme, U. Huebner, H. Schneidewind, M. Zeisberger, R. Mattheis, and J. Popp

Asia Communications and Photonics Conference and Exhibition 2011

Shanghai China
13–16 November 2011
ISBN: 978-0-8194-8959-3

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Biophotonics I (BIOI)

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D. Cialla, K. Weber, R. Boehme, U. Huebner, H. Schneidewind, M. Zeisberger, R. Mattheis, and J. Popp, "Characterization and bioanalytical application of innovative plasmonic nanostructures," in Optical Sensors and Biophotonics, J. Popp, D. Matthews, J. Tian, and C. Yang, eds., Vol. 8311 of Proceedings of SPIE (Optica Publishing Group, 2011), paper 83110J.

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Abstract

To combine fluorescence and surface enhanced Raman spectroscopy (SERS) utilizing the same plasmonic array, a silver deposited micro fabricated quartz grating prepared by electron beam lithography is used. The most efficient Raman signal amplification is achieved for molecules directly adsorbed on the metallic surface, while fluorescence is quenched under these conditions. By careful adjustment, fluorescence is also enhanced which is known as metal enhanced fluorescence. In this contribution, the combined fluorescence and SERS readout is verified by a DNA detection scheme based on the usage of the label molecule ATTO565.

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