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Conferences > OMP > 2017 > OmS2D > OmS2D.5

High-Speed Two-Photon Fluorescence Lifetime Imaging Microscopy of NADH for Label-Free Metabolic Imaging

Andrew J. Bower, Joanne Li, Eric J. Chaney, Marina Marjanovic, and Stephen A. Boppart

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Abstract

Two-photon fluorescence lifetime imaging microscopy (TP-FLIM) of reduced nicotinamide adenine dinucleotide is a sensitive metabolic imaging modality. Here, a video rate TP-FLIM system is demonstrated for metabolic imaging in cells and tissues.

© 2017 Optical Society of America

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