Wide Field-Of-View, Multi-Region Two-Photon Imaging of Neuronal Activity In Vivo

Jeffrey N. Stirman; Jeffrey N. Stirman; Ikuko T. Smith; Ikuko T. Smith; Michael W. Kudenov; Spencer L. Smith; Spencer L. Smith; Spencer L. Smith

doi:10.1364/BRAIN.2016.BTu2D.2

Biomedical Optics 2016
OSA Technical Digest (online) (Optica Publishing Group, 2016),
paper BTu2D.2
•https://doi.org/10.1364/BRAIN.2016.BTu2D.2

Wide Field-Of-View, Multi-Region Two-Photon Imaging of Neuronal Activity In Vivo

Jeffrey N. Stirman, Ikuko T. Smith, Michael W. Kudenov, and Spencer L. Smith

Optics and the Brain 2016

Fort Lauderdale, Florida United States
25–28 April 2016
ISBN: 978-1-943580-10-1

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Functional Brain Microscopy: Improving Depth and Speed (BTu2D)

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J. N. Stirman, I. T. Smith, M. W. Kudenov, and S. L. Smith, "Wide Field-Of-View, Multi-Region Two-Photon Imaging of Neuronal Activity In Vivo," in Biomedical Optics 2016, OSA Technical Digest (online) (Optica Publishing Group, 2016), paper BTu2D.2.

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Abstract

We present a custom two-photon microscope that provides cellular resolution over a 3.5 mm field of view. Temporally multiplexed excitation pathways can be independently positioned in XYZ to simultaneously image regions within the large field of view.

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