Abstract
We introduce a fiber-based laser system providing 130 fs pulses with 3.5 nJ energy at 920 nm at a 43 MHz repetition rate and illustrate the potential of the source for two-photon excited fluorescence microscopy of living mouse brain. The laser source is based on frequency-doubling high-energy solitons generated and frequency-shifted to 1840 nm in large mode area fibers. This simple laser system could unleash the potential of two-photon microscopy techniques in the biology laboratory where green fluorescent proteins with two-photon absorption spectrum peaking around 920 nm are routinely used.
© 2018 Optical Society of America
Full Article | PDF ArticleMore Like This
Duanyang Xu, Konstantinos N. Bourdakos, Anna Crisford, Peter Johnson, Ibrahim Abughazaleh, Panuwat Srisamran, Richard O. C. Oreffo, Sumeet Mahajan, David J. Richardson, and Lin Xu
Biomed. Opt. Express 14(9) 4520-4530 (2023)
H. Delahaye, G. Granger, J.-T. Gomes, L. Lavoute, D. Gaponov, N. Ducros, and S. Fevrier
Opt. Lett. 44(9) 2318-2321 (2019)
Hongsen He, Meng Zhou, Tian Qiao, Hei Ming Lai, Qiao Ran, Yu-Xuan Ren, Ho Ko, Chaogu Zheng, Kevin K. Tsia, and Kenneth K. Y. Wong
Opt. Lett. 47(11) 2710-2713 (2022)