Abstract
We have demonstrated successful two-photon excitation fluorescence bioimaging using a high-power pulsed all-semiconductor laser. Toward this purpose, we developed a pulsed light source consisting of a mode-locked laser diode and a two-stage diode laser amplifier. This pulsed light source provided optical pulses of duration and having a maximum peak power of over 100 W at a wavelength of and a repetition frequency of .
© 2007 Optical Society of America
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