Abstract

Near infrared (NIR) spectroscopy was used to determine the carbon and nitrogen content of compost during compost fermentation of tofu (soybean-curd) refuse. Reflectance measurements in the wavelength range between 400 and 2500 nm were made at 2 nm intervals. The NIR absorption of the carbon components was observed at two wavelengths, 1584 and 1024 nm in the second derivative spectrum. The NIR absorption of nitrogen components was observed at two wavelengths, 2174 and 900 nm in the second derivative spectrum. To formulate a calibration equation, a multiple linear regression analysis was carried out between the NIR spectral data and the carbon and nitrogen content in the calibration sample set (sample number, n = 60) obtained using a Pregie–Dumas combustion method. The values of the multiple correlation coefficient were 0.988 and 0.984, respectively. To validate the calibration equations obtained, the carbon and nitrogen content in the prediction sample set (n = 48), not used for formulating the calibration equation, were calculated using the calibration equations and compared with the values obtained using the Pregie–Dumas combustion method. Good agreement was observed between the results of the Pregie–Dumas combustion method and the NIR method. The values of the simple correlation coefficient were 0.986 and 0.972, respectively. The study indicates that NIR is a useful method for process management of the compost fermentation of tofu refuse.

© 2001 NIR Publications

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