Abstract
Reduced depth of field is a severe limitation in microscopy since, at high
magnification, only a portion of the imaged volume along the optical axis is
in good focus at once. We demonstrate that, adopting a numerical quadratic
deformed diffraction grating into the reconstruction process of digital
holograms, it is possible to image three planes at different depths
simultaneously, gaining flexibility dispensing with a physical grating.
Moreover, multiplexed holograms recorded with two different wavelengths can
be obtained in focus simultaneously by a single reconstruction. Description
of the method and applications for macroscopic and microscopic biological
samples are reported.
© 2011 IEEE
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