Abstract
We recently reported on the use of defocused imaging for single molecule localization microscopy close to the coverslip [Biomed. Opt. Express , 11, 775 (2020) [CrossRef] ]. The original manuscript contains an error in the description of the Cramér-Rao lower bound (CRLB) calculations for SALM/DONALD, which affected the corresponding precision plot in Figure 2(b). We present a corrected description and plot.
© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
In a recent publication we proposed defocused imaging for single molecule localization microscopy (SMLM) close to the coverslip [1]. The description for calculating CRLB-based precision curves for the photometric approach used in SALM/DONALD provided in the appendix contains an error: For SALM/DONALD, the Fisher matrices for the PSFs of the two detection channels (UAF and TOT) should not contain z-derivatives, i.e., the last row and column in the Fisher matrix of Eq. (7) of the paper should not be included. This reflects the assumption that the z position of the molecule does not affect the signal estimates.
The resulting z-precision curve for SALM/DONALD shows improved precisions compared the plot in Figure 2(b) of our manuscript. The corrected curve is shown in Fig. 1 and compared to the z-precision curves of SALM/DONALD+, off-focus imaging ($\Gamma$ = 500 nm) and results from Monte Carlo (MC) simulations for each method (marked by dots, each dot marks the average of 300 estimates).
Acknowledgements
We would like to thank Jonas Ries for a helpful discussion about CRLB calculations.
References
1. P. Zelger, L. Bodner, L. Velas, G. J. Schütz, and A. Jesacher, “Defocused imaging exploits supercritical-angle fluorescence emission for precise axial single molecule localization microscopy,” Biomed. Opt. Express 11(2), 775–790 (2020). [CrossRef]