Abstract

Mid-infrared spectra of spores of two strains of <i>Bacillus subtilis</i>, PS832 (wild-type) and FB122 (<i>sleB spoVF</i>), that are isogenic except for the two mutations in FB122 were obtained by Fourier transform infrared (FT-IR) reflectance microspectroscopy. The mutations in FB122 cause the spores of this strain to be devoid of dipicolinic acid (pyridine-2,6-dicarboxylic acid; DPA), a biomarker characteristic of bacterial spores. Analysis of these two strains by difference spectroscopy revealed a spectrum similar to that of calcium dipicolinate (CaDPA), a chelate salt of DPA. This difference spectrum was compared to mid-infrared spectra of both DPA and CaDPA, and was attributed to CaDPA only. This is the first report known to the authors of a genetically engineered organism being used to identify the spectral contribution of a particular cellular component.

Two-dimensional multiwavelength fluorescence spectra of dipicolinic acid and calcium dipicolinate

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Proteins and dipicolinic acid released during heat shock activation of Bacillus subtilis spores probed by optical spectroscopy

Alexandra Alimova, A. Katz, Paul Gottlieb, and R. R. Alfano
Appl. Opt. 45(3) 445-450 (2006)

Probing the germination kinetics of ethanol-treated Bacillus thuringiensis spores

Guiwen Wang, Huanjun Chen, Xiaochun Wang, Lixin Peng, Yuan Peng, and Yong-qing Li
Appl. Opt. 56(12) 3263-3269 (2017)

Native fluorescence and excitation spectroscopic changes in Bacillus subtilis and Staphylococcus aureus bacteria subjected to conditions of starvation

Alexandra Alimova, Alvin Katz, Howard E. Savage, Mahendra Shah, Glenn Minko, Daniel V. Will, Richard B. Rosen, Steven A. McCormick, and Robert R. Alfano
Appl. Opt. 42(19) 4080-4087 (2003)

Characterization of single heat-activated Bacillus spores using laser tweezers Raman spectroscopy

Pengfei Zhang, Peter Setlow, and Yongqing Li
Opt. Express 17(19) 16480-16491 (2009)