Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 65,
  • Issue 2,
  • pp. 206-215
  • (2011)

Portable, Fiber-Based, Diffuse Reflection Spectroscopy (DRS) Systems for Estimating Tissue Optical Properties

Not Accessible

Your library or personal account may give you access

Abstract

Steady-state diffuse reflection spectroscopy is a well-studied optical technique that can provide a noninvasive and quantitative method for characterizing the absorption and scattering properties of biological tissues. Here, we compare three fiber-based diffuse reflection spectroscopy systems that were assembled to create a light-weight, portable, and robust optical spectrometer that could be easily translated for repeated and reliable use in mobile settings. The three systems were built using a broadband light source and a compact, commercially available spectrograph. We tested two different light sources and two spectrographs (manufactured by two different vendors). The assembled systems were characterized by their signal-to-noise ratios, the source-intensity drifts, and detector linearity. We quantified the performance of these instruments in extracting optical properties from diffuse reflectance spectra in tissue-mimicking liquid phantoms with well-controlled optical absorption and scattering coefficients. We show that all assembled systems were able to extract the optical absorption and scattering properties with errors less than 10%, while providing greater than ten-fold decrease in footprint and cost (relative to a previously well-characterized and widely used commercial system). Finally, we demonstrate the use of these small systems to measure optical biomarkers in vivo in a small-animal model cancer therapy study. We show that optical measurements from the simple portable system provide estimates of tumor oxygen saturation similar to those detected using the commercial system in murine tumor models of head and neck cancer.

PDF Article
More Like This
Diffuse reflectance spectroscopy of epithelial tissue with a smart fiber-optic probe

Bing Yu, Amy Shah, Vivek K. Nagarajan, and Daron G. Ferris
Biomed. Opt. Express 5(3) 675-689 (2014)

Tissue-mimicking phantom materials with tunable optical properties suitable for assessment of diffuse reflectance spectroscopy during electrosurgery

Sara Azizian Amiri, Pieter Van Berckel, Marco Lai, Jenny Dankelman, and Benno H. W. Hendriks
Biomed. Opt. Express 13(5) 2616-2643 (2022)

Evaluating differences in optical properties of indolent and aggressive murine breast tumors using quantitative diffuse reflectance spectroscopy

Joel Rodriguez Troncoso, Umme Marium Mim, Jesse D. Ivers, Santosh K. Paidi, Mason G. Harper, Khue G. Nguyen, Sruthi Ravindranathan, Lisa Rebello, David E. Lee, David A. Zaharoff, Ishan Barman, and Narasimhan Rajaram
Biomed. Opt. Express 14(12) 6114-6126 (2023)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved