Abstract
An underwater optical serial-sectioning technique is developed to measure in situ three-dimensional distributions of biological particles. The technique involves scanning of a thin plane of laser light through a range of distances parallel to the imaging plane of a digital CCD camera. Images of induced fluorescence in the sequentially illuminated planes are recorded. An inverse method is then used to reconstruct three-dimensional chlorophyll a distributions from the plane images. Computer simulations of the image formation and reconstruction process indicate that the underwater optical serial-sectioning technique is practical for in situ determination and analysis of chlorophyll a microstructures for concentrations as low as 0.1 mg of chlorophyll a per cubic meter in 1-m3 water volumes.
© 1994 Optical Society of America
Full Article | PDF ArticleMore Like This
Timothy J. Cowles, James N. Moum, Russell A. Desiderio, and Stanley M. Angel
Appl. Opt. 28(3) 595-600 (1989)
George W. Kattawar and John C. Vastano
Appl. Opt. 21(14) 2489-2492 (1982)
Frank E. Hoge, Paul E. Lyon, C. Wayne Wright, Robert N. Swift, and James K. Yungel
Appl. Opt. 44(14) 2857-2862 (2005)