Abstract
A technique is proposed for determination of the three-dimensional orientation of the transition dipoles of single molecules by use of polarization-sensitive detection of fluorescence through a high-N.A. objective. Molecular orientation can be determined uniquely and rapidly based on the counts from three detectors that are sensitive to different polarizations of light.
© 2001 Optical Society of America
Full Article | PDF ArticleMore Like This
Matthew R. Foreman, Carlos Macías Romero, and Peter Török
Opt. Lett. 33(9) 1020-1022 (2008)
Zbigniew Sikorski and Lloyd M. Davis
Opt. Express 16(6) 3660-3673 (2008)
M. Andreas Lieb, James M. Zavislan, and Lukas Novotny
J. Opt. Soc. Am. B 21(6) 1210-1215 (2004)