Abstract
Amygdalin is a cyanogenic glycoside found in the seeds of several plants
belonging to the Rosaceae family. Cyanogenic glycosides can be specifically probed
by Raman spectroscopy due to an inherent nitrile group which shows a well-resolved
band near 2245 cm−1. In the current study the subcellular distribution of
amygdalin in thin apricot (Prunus armeniaca) seed sections is probed by high-resolution Raman imaging with a
step size of 2.5 μm. Further, Raman images and line maps were collected from four
apricot seeds with step sizes between 30 and 70 μm. The data were processed by
functional group mapping and the spectral unmixing algorithm vertex component
analysis. Spectral contributions of amygdalin, lipids, and cellulose were
identified. One seed had low amygdalin content in its center and higher content
toward its epidermis. The other three specimens showed different distributions of
amygdalin, with highest concentration in the center and local concentration spots
throughout the seed. We conclude from these preliminary results on Raman imaging in
apricot seeds that amygdalin is unevenly distributed and its location does not
follow the same pattern for all seeds. The observed biological variability of the
amygdalin distribution cannot yet be explained satisfactorily and requires further
investigation.
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