Abstract
After discussion of several technical problems regarding instrumentation of fluorescence microscopy and of the ways how to solve them, a device is described which makes it possible to examine fluorescent specimens both in positive and negative phase contrast (so-called simultaneous contrast fluorescence). Furthermore, light filtration and the relating questions are briefly mentioned. Finally, the resulting effects and the manifold fields of application of this new device are demonstrated with the aid of two examples and its advantages are discussed.
© 1965 Optical Society of America
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