Abstract
Multiphoton microscopy has been developed as a powerful technique for high resolution fluorescence imaging due to the development of broadband ultrashort light pulses [1]. The capability of a simple and reproducible setup to obtain broadband pulses has several important biological applications. For example, the efficiency of the nonlinear excitation process can be improved by tailoring the amplitude and phase of the pulses that interact with the biological sample. This technique requires a complete characterization of the pulses at the sample plane. Here we report the generation of controlled broadband pulses using the output pulses of a Ti:sapphire laser (ΔT ~ 180 fs and spectral width Δλ ~ 10 nm) traveling through a bending insensitive single mode fiber (OFS Micro820-16 Fiber) with a length of L=10cm. A pair of prisms (SF10) is used to control the chirp of the pulses from the fiber. The pulses are then sent to an adapted inverted Nikon TE2000-U microscope and are fully characterized at the sample plane of the microscope using the MEFISTO technique (measurement of electrical field by interferometric spectral trace observation) [2].
© 2007 IEEE
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