Abstract
We present a fiber-optic low-coherence imaging technique, termed spectral-domain differential interference contrast microscopy (SD-DIC), for quantitative DIC imaging of both reflective surfaces and transparent biological specimens. SD-DIC combines the common-path nature of a Nomarski DIC interferometer with the high sensitivity of spectral-domain low-coherence interferometry to obtain high-resolution, quantitative measurements of optical pathlength gradients from a single point on the sample. Full-field imaging can be achieved by scanning the sample. A reflected-light SD-DIC system was demonstrated using a USAF resolution target as the phase object. Live cardiomyocytes were also imaged, achieving a resolution of for pathlength gradient measurements. The dynamics of cardiomyocyte contraction were recorded with high sensitivity at selected sites on the cells.
© 2011 Optical Society of America
Full Article | PDF ArticleMore Like This
Chulmin Joo, Taner Akkin, Barry Cense, Boris H. Park, and Johannes F. de Boer
Opt. Lett. 30(16) 2131-2133 (2005)
Michael Shribak and Shinya Inoué
Appl. Opt. 45(3) 460-469 (2006)
Jianling Chen, Yan Xu, Xiaohua Lv, Xiaomin Lai, and Shaoqun Zeng
Opt. Express 21(1) 112-121 (2013)