Abstract
A laser is commonly used for biological optical trapping. However, it has the problem of generating reactive oxygen species in the presence of a sensitizer, which leads to photo damage in biological samples. Here we constructed optical tweezers using a tapered amplifier diode laser that operates at . Compared to a laser, this laser is friendly to live cells, eliminates photo damage associated with reactive oxygen species, and allows simultaneous two-photon fluorescence imaging of green fluorescent proteins in live mammalian cells. All these advantages could significantly benefit future application of this single molecule technique in biological studies.
© 2010 Optical Society of America
Full Article | PDF ArticleMore Like This
Ximiao Hou and Wei Cheng
Opt. Lett. 36(16) 3185-3187 (2011)
Pengfei Zhang, Lingbo Kong, Peter Setlow, and Yong-qing Li
Opt. Lett. 35(20) 3321-3323 (2010)
Mingjun Chi, G. Erbert, B. Sumpf, and Paul Michael Petersen
Opt. Lett. 35(10) 1545-1547 (2010)