Abstract

Pupil filters are widely used to improve the resolution of confocal microscopes. We analyze the possibilities of applying them to N-photon microscopy. We find that taking a linear combination of images obtained with several pupil filters can improve the resolution by a factor of N (compared to a conventional microscope). When applied to saturable fluorescence, this technique allows one to observe fluorescent objects with, in principle, unlimited spatial resolution.

© 2009 Optical Society of America

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