Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Imaging subcellular scattering contrast by using combined optical coherence and multiphoton microscopy

Not Accessible

Your library or personal account may give you access

Abstract

The structural origin of scattering contrast from single cells is examined by using a combined optical coherence and multiphoton microscope based on a 12fs Ti:sapphire source and a 0.95 NA objective. High-resolution coherence-gated scattering images from single cells are coregistered and compared with two-photon-excited fluorescence images. Scattering contrast is observed from mitochondria, plasma membrane, actin filaments, and the boundary between cytoplasm and nucleus. There is little contribution to scattering from regions inside the nuclear core. These results confirm that light scattering signals from specific subcellular structures can be visualized by using coherent reflectance geometry.

© 2007 Optical Society of America

Full Article  |  PDF Article
More Like This
Spectral-domain optical coherence phase and multiphoton microscopy

Chulmin Joo, Ki Hean Kim, and Johannes F. de Boer
Opt. Lett. 32(6) 623-625 (2007)

Multiscale multimodal imaging with multiphoton microscopy and optical coherence tomography

Shuo Tang, Yifeng Zhou, Kenny K. H. Chan, and Tom Lai
Opt. Lett. 36(24) 4800-4802 (2011)

Unified Mie and fractal scattering by biological cells and subcellular structures

Tao T. Wu, Jianan Y. Qu, and Min Xu
Opt. Lett. 32(16) 2324-2326 (2007)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (2)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved