Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Separation of phase and absorption images in phase-contrast microscopy

Not Accessible

Your library or personal account may give you access

Abstract

A method for separating the phase distribution from the image of a semitransparent sample observed with a phase-contrast microscope is proposed. Three images of the same sample are used for calculating the phase distribution, one observed by a normal objective lens and the others observed by a bright-contrast objective and a dark-contrast objective. The method works even for samples that are not weak phase objects. The phase (or refractive-index) distribution is quantitatively reconstructed by numerically solving a set of linear equations for every pixel of the sample image. The method of phase reconstruction is mathematically derived and is explained with graphs in the complex amplitude space. Experimental results are also presented.

© 1992 Optical Society of America

Full Article  |  PDF Article
More Like This
Methods of improving the images in phase-contrast microscopy: theory and computer simulation

Iku Nemoto and Akira Takahashi
J. Opt. Soc. Am. A 8(3) 511-519 (1991)

X-ray phase-contrast imaging: transmission functions separable in Cartesian coordinates

Guohua Cao, Theron J. Hamilton, Christoph Rose-Petruck, and Gerald J. Diebold
J. Opt. Soc. Am. A 24(4) 1201-1208 (2007)

Image Contrast in Phase-Contrast Microscopy

Paul H. Keck and Arthur T. Brice
J. Opt. Soc. Am. 39(7) 507-514 (1949)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (16)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (21)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved