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Improving the axial and lateral resolution of three-dimensional fluorescence microscopy using random speckle illuminations

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Abstract

We consider a fluorescence microscope in which several three-dimensional images of a sample are recorded for different speckle illuminations. We show, on synthetic data, that by summing the positive deconvolution of each speckle image, one obtains a sample reconstruction with axial and transverse resolutions that compare favorably to that of an ideal confocal microscope.

© 2016 Optical Society of America

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