Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Chinese Optics Letters
  • Vol. 2,
  • Issue 3,
  • pp. 160-161
  • (2004)

Characteristics of blood fluorescence spectra using low-level, 457.9-nm excitation from Ar+ laser

Not Accessible

Your library or personal account may give you access

Abstract

We measured the fluorescence spectra of the whole blood, the red blood cell (RBC) and the hemoglobin using 457.9-nm Ar+ laser excitation. It was found that the fluorescence spectra of the whole blood and the RBC have much similarities in the intensity, the emission peaks and the emitting region, and abundant peaks can be found. But for the hemoglobin, fluorescence could only be found in the wavelength range 580-650 nm. It was concluded that in the wavelength range of 650-850 nm, the fluorescence spectra were emitted by the new fluorophores generated by the breakdown of some weak bonds on the RBC membrane, such as the C-C bond and the C-N bond. In the wavelength range of 590-650 nm, the fluorescence spectra are mainly emitted by the hemoglobin, but the hemoglobin solution of cracked RBC has a strong quencher effect on the fluorescence spectrum. The experimental result and the theoretical analysis are meaningful for the medical diagnostics and the therapy.

© 2005 Chinese Optics Letters

PDF Article
More Like This
Multiphoton excited hemoglobin fluorescence and third harmonic generation for non-invasive microscopy of stored blood

Ilyas Saytashev, Rachel Glenn, Gabrielle A. Murashova, Sam Osseiran, Dana Spence, Conor L. Evans, and Marcos Dantus
Biomed. Opt. Express 7(9) 3449-3460 (2016)

Two-photon excited hemoglobin fluorescence

Wei Zheng, Dong Li, Yan Zeng, Yi Luo, and Jianan Y. Qu
Biomed. Opt. Express 2(1) 71-79 (2011)

Spectroscopic properties of various blood antigens/antibodies

Seyedeh Solaleh Seyedi, Parviz Parvin, Amir Jafargholi, Nazanin Hashemi, Seyed Morteza Tabatabaee, Ali Abbasian, and Ahmad Khorrami
Biomed. Opt. Express 11(4) 2298-2312 (2020)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved