Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 59,
  • Issue 1,
  • pp. 1-9
  • (2005)

Evaluation of Toxic Agent Effects on Lung Cells by Fiber Evanescent Wave Spectroscopy

Not Accessible

Your library or personal account may give you access

Abstract

Biochemical changes in living cells are detected using a fiber probe system composed of a single chalcogenide fiber acting as both the sensor and transmission line for infrared optical signals. The signal is collected via evanescent wave absorption along the tapered sensing zone of the fiber. We spectroscopically monitored the effects of the surfactant Triton X-100, which serves as a toxic agent simulant on a transformed human lung carcinoma type II epithelial cell line (A549). We observe spectral changes between 2800–3000 cm<sup>-1</sup> in four absorptions bands, which are assigned to hydrocarbon vibrations of methylene and methyl groups in membrane lipids. Comparison of fiber and transmission spectra shows that the present technique allows one to locally probe the cell plasma membrane in the lipid spectral region. These optical responses are correlated with cellular metabolic activity measurements and LDH (lactate dehydrogenase) release assays that indicate a loss of cellular function and membrane integrity as would be expected in response to the membrane solubilizing Triton. The spectroscopic technique shows a significantly greater detection resolution in time and concentration.

PDF Article
More Like This
Evaluating the toxic effect of an antimicrobial agent on single bacterial cells with optical tweezers

Akbar Samadi, Chensong Zhang, Joseph Chen, S. N. S. Reihani, and Zhigang Chen
Biomed. Opt. Express 6(1) 112-117 (2015)

Quantitative evaluation of chalcogenide glass fiber evanescent wave spectroscopy

M. Katz, A. Katzir, I. Schnitzer, and A. Bornstein
Appl. Opt. 33(25) 5888-5894 (1994)

Anti-cancer effect of bee venom on human MDA-MB-231 breast cancer cells using Raman spectroscopy

Gyeong Bok Jung, Jeong-Eun Huh, Hyo-Jung Lee, Dohyun Kim, Gi-Ja Lee, Hun-Kuk Park, and Jae-Dong Lee
Biomed. Opt. Express 9(11) 5703-5718 (2018)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved