Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 55,
  • Issue 6,
  • pp. 670-678
  • (2001)

Multiple Time-Gate Module for Fluorescence Lifetime Imaging

Not Accessible

Your library or personal account may give you access

Abstract

A versatile multiple time-gate fluorescence lifetime acquisition module is presented. The module is used as an add-on to a two-photon scanning laser microscope equipped with pulsed excitation for fluorescence lifetime imaging. It enables the recording of fluorescence lifetimes by capturing the intensity decay in four or eight time gates. The module allows fluorescence lifetime imaging at high count rates and with a high efficiency. The module is equipped with computer-controlled variable-delay lines for setting the gate widths. Decay times can be accurately measured in the 0.5 ns–1 μs range. Fluorescence lifetime imaging experiments on a number of single dye solutions with different (mono-exponential) fluorescence lifetimes are in excellent agreement with the reference values obtained with time-correlated single photon counting. Measurements on a test specimen exhibiting a bi-exponential decay demonstrate that the two decay components can be quantified with high accuracy. In a specimen containing six different lifetimes, in the range 2.8 to 12 ns, all lifetimes could be well distinguished. As an example, the results of a pH imaging experiment on oral biofilm using carboxyfluorescein are presented. Here, fluorescence lifetime imaging is used both to separate the short-lifetime autofluorescence from the carboxy-fluorescein fluorescence and to determine the pH in the biofilm.

PDF Article
More Like This
In vitro and in vivo NIR fluorescence lifetime imaging with a time-gated SPAD camera

Jason T. Smith, Alena Rudkouskaya, Shan Gao, Juhi M. Gupta, Arin Ulku, Claudio Bruschini, Edoardo Charbon, Shimon Weiss, Margarida Barroso, Xavier Intes, and Xavier Michalet
Optica 9(5) 532-544 (2022)

High-speed wide-field time-gated endoscopic fluorescence-lifetime imaging

J. Requejo-Isidro, J. McGinty, I. Munro, D. S. Elson, N. P. Galletly, M. J. Lever, M. A. A. Neil, G. W. H. Stamp, P. M. W. French, P. A. Kellett, J. D. Hares, and A. K. L. Dymoke-Bradshaw
Opt. Lett. 29(19) 2249-2251 (2004)

Dead-time correction of fluorescence lifetime measurements and fluorescence lifetime imaging

Sebastian Isbaner, Narain Karedla, Daja Ruhlandt, Simon Christoph Stein, Anna Chizhik, Ingo Gregor, and Jörg Enderlein
Opt. Express 24(9) 9429-9445 (2016)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved