Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 48,
  • Issue 3,
  • pp. 400-405
  • (1994)

Error Analysis of Simple Algorithms for Determining Fluorescence Lifetimes in Ultradilute Dye Solutions

Not Accessible

Your library or personal account may give you access

Abstract

We have evaluated the use of two simple algorithms for determining the decay parameters describing a single exponential process for dyes with nanosecond and subnanosecond fluorescence lifetimes in the limit of low concentrations and high backgrounds from scattered photons generated by the solvent using experimental and Monte Carlo simulation results. These algorithms, the maximum likelihood estimator (MLE) and the rapid lifetime determination (RLD), are computationally easy to perform, allowing the evaluation of large amounts of data quickly and efficiently. The MLE and RLD methods were used to calculate the fluorescence lifetimes of three near-IR dyes with lifetimes spanning the range of 0.57 ns to 1.12 ns. For low-concentration conditions and high background-to-fluorescence ratios, the MLE method resulted in larger errors when compared to RLD, although both methods yielded comparable standard deviations. However, when the interval over which the lifetime was calculated within the decay profile was shifted to latter times in order to reduce the amount of scattered photons included in the calculation, significant improvements in the accuracy were observed with the use of MLE. Shifting the start channel of the calculation to latter time channels within the decay profile did not affect the lifetime with the use of RLD. Inclusion of large amounts of scattering photons was found to bias the calculated lifetime to lower values, reducing the accuracy of the determination. The relative standard deviations for MLE and RLD were found to be approximately 2-3% at a background-to-fluorescence ratio of 0.5. The absolute relative error in the methods at the 0.50 background-to-fluorescence ratio ranged from 14 to 27% for MLE and 8 to 18% for the RLD method when the calculation was initiated at t = 0. This error was found to decrease to < 1% with the use of MLE when the calculation was initiated at t ≈ 100 ps.

PDF Article
More Like This
On-chip, time-correlated, fluorescence lifetime extraction algorithms and error analysis

Day-Uei Li, Eleanor Bonnist, David Renshaw, and Robert Henderson
J. Opt. Soc. Am. A 25(5) 1190-1198 (2008)

Calibration method for the center of mass method to enlarge the solvable range of fluorescence lifetime

Jiangtao Xu, Jun Qiao, Kaiming Nie, and An Zhang
J. Opt. Soc. Am. A 33(10) 1961-1969 (2016)

Hardware implementation and calibration of background noise for an integration-based fluorescence lifetime sensing algorithm

Day-Uei Li, Richard Walker, Justin Richardson, Bruce Rae, Alex Buts, David Renshaw, and Robert Henderson
J. Opt. Soc. Am. A 26(4) 804-814 (2009)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved