Abstract

A compact, portable spectrofluorimeter which measures broad-band fluorophores with high sensitivity is described. This instrument is specifically designed for acquisition of human tissue autofluorescence spectra <i>in vivo.</i> Pulsed excitation laser light at 337 nm and in the 370-700 nm wavelength range is provided, and the resulting fluorescence can be measured with 10-nm resolution. The system produces single-pulse spectra in human artery wall tissues with a signal-to-noise ratio of 100:1. An optically shielded fiber-optic probe for light delivery and collection permits calibrated intensity information to be obtained.

Rapid multiexcitation fluorescence spectroscopy system for in vivo tissue diagnosis

Renato Amaro Zângaro, Landulfo Silveira, Ramasamy Manoharan, George Zonios, Irving Itzkan, Ramachandra R. Dasari, Jacques Van Dam, and Michael S. Feld
Appl. Opt. 35(25) 5211-5219 (1996)

Dynamic tissue analysis using time- and wavelength-resolved fluorescence spectroscopy for atherosclerosis diagnosis

Yinghua Sun, Yang Sun, Douglas Stephens, Hongtao Xie, Jennifer Phipps, Ramez Saroufeem, Jeffrey Southard, Daniel S. Elson, and Laura Marcu
Opt. Express 19(5) 3890-3901 (2011)

Characterization of human cutaneous tissue autofluorescence: implications in topical drug delivery studies with fluorescence microscopy

Maiko Hermsmeier, Sinyoung Jeong, Akira Yamamoto, Xin Chen, Usha Nagavarapu, Conor L. Evans, and Kin F. Chan
Biomed. Opt. Express 9(11) 5400-5418 (2018)

Standardization of Fluorescence Spectra and the Calibration of Spectrofluorimeters

J. W. Eastman
Appl. Opt. 5(7) 1125-1132 (1966)

Video-rate confocal scanning laser microscope for imaging human tissues in vivo

Milind Rajadhyaksha, R. Rox Anderson, and Robert H. Webb
Appl. Opt. 38(10) 2105-2115 (1999)