Abstract
The multiphoton fluorescence excitation observed in a continuous-wave (cw) single-beam gradient force optical trap (optical tweezers) is reported for latex beads labeled with a yellow-green fluorescent dye (BODIPY). The fluorescence emission spectra of the yellow-green beads trapped and excited by the same 1064-nm laser light are identical to the spectra excited by the 365-nm UV light. The influence of the numerical aperture of the objective on the slope of the log–log power-dependence has been demonstrated for BODIPY–Oil solution under cw and pulsed-laser conditions. The possibility that three-photon excitation process occurs is discussed within the context of a dog-bone saturation model. Other possibilities for the observed fluorescence excitation have been discussed.
© 1998 Optical Society of America
Full Article | PDF ArticleMore Like This
Stefan W. Hell, Martin Booth, Stefan Wilms, Christoph M. Schnetter, Achim K. Kirsch, Donna J. Arndt-Jovin, and Thomas M. Jovin
Opt. Lett. 23(15) 1238-1240 (1998)
Y. Liu, G. J. Sonek, M. W. Berns, K. Konig, and B. J. Tromberg
Opt. Lett. 20(21) 2246-2248 (1995)
Mattias Goksör, Jonas Enger, and Dag Hanstorp
Appl. Opt. 43(25) 4831-4837 (2004)