Abstract
A portable fluorescence optical detection system was developed to demonstrate real-time in situ analysis of cells that are three-dimensionally cultured in an extracellular matrix under microfluidic environment. The system was designed to provide a large field of view in the lateral plane to average out cellular processes in an axial layer and simultaneously diffraction-limited axial resolution. In this proof-of-concept study, the detection system was applied to quantitative analyses of short-term measurements of cell staining and cell cytotoxicity and long-term monitoring of a cell-invasion assay. For assays, colon cancer cells were cultured in a Matrigel or alginate matrix. The measured data were largely consistent with predicted results and revealed quantitatively cell dynamics specific to 3D cell cultures. The detection system has a potential as a single package to investigate 3D cultures in a microfluidic system.
© 2010 Optical Society of America
Full Article | PDF ArticleMore Like This
Daniel A. Tatosian, Michael L. Shuler, and Donghyun Kim
Opt. Lett. 30(13) 1689-1691 (2005)
Julie C. McIntosh, Lin Yang, Ting Wang, Haibo Zhou, Matthew R. Lockett, and Amy L. Oldenburg
Biomed. Opt. Express 11(6) 3181-3194 (2020)
Woo June Choi, Do In Jeon, Sang-Gun Ahn, Jung-Hoon Yoon, Sungho Kim, and Byeong Ha Lee
Opt. Express 18(22) 23285-23295 (2010)